On display are works by great Impressionists (Manet, Cassatt, Morisot, Monet, Pissarro, Sisley), Nabis (Bonnard, Maurice Denis, Vuillard), Post-Impressionists (Chagall, Dufy, Rouault, Vlaminck) as well as modern and contemporary artists (Picasso, Léger, Matisse, Calder, Tanguy, Brauner, Magritte, Dubuffet, Soulages, Warhol, Hockney and Basquiat).

Prominent on the international art market, the gallery exhibits at the most important art fairs, including the Biennale des Antiquaires (Paris), International Fine Art Fair (New York), TEFAF-Maastricht, Art Basel (Basel and Miami Beach). A co-organizer of the Pavillon des Antiquaires et des Beaux-Arts (Paris) since 1998, the gallery has devoted its booth to retrospectives of artists such as Henry Moore, Alexander Calder and Jean Dubuffet.

The Galerie Hopkins-Custot often lends works for major exhibitions, such as those put on by the Metropolitan Museum (New York), the Musée des Beaux-Arts (Montreal) and the Fondation Pierre Gianadda (Martigny, Switzerland). It has also earned recognition from the greatest museums (e.g. the Musée d’Orsay, the Van Gogh Museum in Amsterdam, the Art Institute of Chicago, the Cleveland Museum, the Getty Museum) who have purchased works from Hopkins-Custot for their collections. In 1994, the Dixon Gallery and Gardens in Memphis, Tennessee acquired the gallery's entire Forain collection.

The Galerie Hopkins-Custot's exhibition history illustrates a deliberate play on contrasts and includes a Berthe Morisot retrospective in 1987 (the gallery published a new edition of the Morisot catalogue raisonné) as well as shows of drawings, paintings and sculptures by Fernando Botero in 2001 and 2004, bronze sculptures by contemporary sculptor Jedd Novatt in 2001 and photographs by Constantin Brancusi in 2003.

Since 2006, the Galerie Hopkins-Custot also represents artists Sam Szafran and Marc Quinn.